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lncrna high throughput sequencing analysis  (Annoroad Gene Technology Co Ltd)

 
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    Structured Review

    Annoroad Gene Technology Co Ltd lncrna high throughput sequencing analysis
    (A) Length distribution trend of the samples in the three groups. The average length of each sample is >500 bp. (B) Novel <t>long</t> <t>non-coding</t> <t>RNA</t> exon number analysis in the S1, S2 and S3 groups. Different colors represent different numbers of exons of each transcript. The numbers in the y axis indicate the number of transcripts. (C) Distribution of gene expression. (D) Distribution of sample conservation scores. S1, normal eyes; S2, nuclear cataract; S3, posterior capsule cataract; RPKM, reads per kilobase million mapped reads.
    Lncrna High Throughput Sequencing Analysis, supplied by Annoroad Gene Technology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lncrna+high+throughput+sequencing+analysis/lncrna+sequencing/pmc05716429-76-0-16
    Average 90 stars, based on 1 article reviews
    lncrna high throughput sequencing analysis - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "High-throughput sequencing reveals novel lincRNA in age-related cataract"

    Article Title: High-throughput sequencing reveals novel lincRNA in age-related cataract

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2017.3185

    (A) Length distribution trend of the samples in the three groups. The average length of each sample is >500 bp. (B) Novel long non-coding RNA exon number analysis in the S1, S2 and S3 groups. Different colors represent different numbers of exons of each transcript. The numbers in the y axis indicate the number of transcripts. (C) Distribution of gene expression. (D) Distribution of sample conservation scores. S1, normal eyes; S2, nuclear cataract; S3, posterior capsule cataract; RPKM, reads per kilobase million mapped reads.
    Figure Legend Snippet: (A) Length distribution trend of the samples in the three groups. The average length of each sample is >500 bp. (B) Novel long non-coding RNA exon number analysis in the S1, S2 and S3 groups. Different colors represent different numbers of exons of each transcript. The numbers in the y axis indicate the number of transcripts. (C) Distribution of gene expression. (D) Distribution of sample conservation scores. S1, normal eyes; S2, nuclear cataract; S3, posterior capsule cataract; RPKM, reads per kilobase million mapped reads.

    Techniques Used: Gene Expression

    (A) Upregulated-lncRNA network and sub-networks obtained using the HPRD network. The red and green triangles indicate the position of the networks shown in (B and C). (B) An example of a sub-network of the HPRD network. (C) The network of genes associated with age-related cataract extracted from (A). lncRNA, long non-coding RNA; HPRD, Human Protein Reference Database.
    Figure Legend Snippet: (A) Upregulated-lncRNA network and sub-networks obtained using the HPRD network. The red and green triangles indicate the position of the networks shown in (B and C). (B) An example of a sub-network of the HPRD network. (C) The network of genes associated with age-related cataract extracted from (A). lncRNA, long non-coding RNA; HPRD, Human Protein Reference Database.

    Techniques Used:

    Downregulated mRNA and corresponding long non-coding RNA networks obtained using the Human Protein Reference Database.
    Figure Legend Snippet: Downregulated mRNA and corresponding long non-coding RNA networks obtained using the Human Protein Reference Database.

    Techniques Used:

    Validation of sequencing results by RT-qPCR.
    Figure Legend Snippet: Validation of sequencing results by RT-qPCR.

    Techniques Used: Biomarker Discovery, Sequencing, Next-Generation Sequencing

    Related Articles

    Next-Generation Sequencing:

    Article Title: High-throughput sequencing reveals novel lincRNA in age-related cataract
    Article Snippet: The yield of RNA was determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Inc.). .. LncRNA high throughput sequencing analysis, including labelling, hybridization, scanning, normalization and data analysis, was performed by Annoroad Geomics (Beijing, China) ( , ). .. A quality check of the input total RNA was performed to confirm its integrity by running an aliquot on an Agilent Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA), and the concentration of the RNA was measured using an ultra-micro spectrophotometer.

    Hybridization:

    Article Title: High-throughput sequencing reveals novel lincRNA in age-related cataract
    Article Snippet: The yield of RNA was determined using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Inc.). .. LncRNA high throughput sequencing analysis, including labelling, hybridization, scanning, normalization and data analysis, was performed by Annoroad Geomics (Beijing, China) ( , ). .. A quality check of the input total RNA was performed to confirm its integrity by running an aliquot on an Agilent Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA), and the concentration of the RNA was measured using an ultra-micro spectrophotometer.



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    (A) Length distribution trend of the samples in the three groups. The average length of each sample is >500 bp. (B) Novel <t>long</t> <t>non-coding</t> <t>RNA</t> exon number analysis in the S1, S2 and S3 groups. Different colors represent different numbers of exons of each transcript. The numbers in the y axis indicate the number of transcripts. (C) Distribution of gene expression. (D) Distribution of sample conservation scores. S1, normal eyes; S2, nuclear cataract; S3, posterior capsule cataract; RPKM, reads per kilobase million mapped reads.
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    (A) Length distribution trend of the samples in the three groups. The average length of each sample is >500 bp. (B) Novel <t>long</t> <t>non-coding</t> <t>RNA</t> exon number analysis in the S1, S2 and S3 groups. Different colors represent different numbers of exons of each transcript. The numbers in the y axis indicate the number of transcripts. (C) Distribution of gene expression. (D) Distribution of sample conservation scores. S1, normal eyes; S2, nuclear cataract; S3, posterior capsule cataract; RPKM, reads per kilobase million mapped reads.
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    Image Search Results


    (A) Length distribution trend of the samples in the three groups. The average length of each sample is >500 bp. (B) Novel long non-coding RNA exon number analysis in the S1, S2 and S3 groups. Different colors represent different numbers of exons of each transcript. The numbers in the y axis indicate the number of transcripts. (C) Distribution of gene expression. (D) Distribution of sample conservation scores. S1, normal eyes; S2, nuclear cataract; S3, posterior capsule cataract; RPKM, reads per kilobase million mapped reads.

    Journal: International Journal of Molecular Medicine

    Article Title: High-throughput sequencing reveals novel lincRNA in age-related cataract

    doi: 10.3892/ijmm.2017.3185

    Figure Lengend Snippet: (A) Length distribution trend of the samples in the three groups. The average length of each sample is >500 bp. (B) Novel long non-coding RNA exon number analysis in the S1, S2 and S3 groups. Different colors represent different numbers of exons of each transcript. The numbers in the y axis indicate the number of transcripts. (C) Distribution of gene expression. (D) Distribution of sample conservation scores. S1, normal eyes; S2, nuclear cataract; S3, posterior capsule cataract; RPKM, reads per kilobase million mapped reads.

    Article Snippet: LncRNA high throughput sequencing analysis, including labelling, hybridization, scanning, normalization and data analysis, was performed by Annoroad Geomics (Beijing, China) ( , ).

    Techniques: Gene Expression

    (A) Upregulated-lncRNA network and sub-networks obtained using the HPRD network. The red and green triangles indicate the position of the networks shown in (B and C). (B) An example of a sub-network of the HPRD network. (C) The network of genes associated with age-related cataract extracted from (A). lncRNA, long non-coding RNA; HPRD, Human Protein Reference Database.

    Journal: International Journal of Molecular Medicine

    Article Title: High-throughput sequencing reveals novel lincRNA in age-related cataract

    doi: 10.3892/ijmm.2017.3185

    Figure Lengend Snippet: (A) Upregulated-lncRNA network and sub-networks obtained using the HPRD network. The red and green triangles indicate the position of the networks shown in (B and C). (B) An example of a sub-network of the HPRD network. (C) The network of genes associated with age-related cataract extracted from (A). lncRNA, long non-coding RNA; HPRD, Human Protein Reference Database.

    Article Snippet: LncRNA high throughput sequencing analysis, including labelling, hybridization, scanning, normalization and data analysis, was performed by Annoroad Geomics (Beijing, China) ( , ).

    Techniques:

    Downregulated mRNA and corresponding long non-coding RNA networks obtained using the Human Protein Reference Database.

    Journal: International Journal of Molecular Medicine

    Article Title: High-throughput sequencing reveals novel lincRNA in age-related cataract

    doi: 10.3892/ijmm.2017.3185

    Figure Lengend Snippet: Downregulated mRNA and corresponding long non-coding RNA networks obtained using the Human Protein Reference Database.

    Article Snippet: LncRNA high throughput sequencing analysis, including labelling, hybridization, scanning, normalization and data analysis, was performed by Annoroad Geomics (Beijing, China) ( , ).

    Techniques:

    Validation of sequencing results by RT-qPCR.

    Journal: International Journal of Molecular Medicine

    Article Title: High-throughput sequencing reveals novel lincRNA in age-related cataract

    doi: 10.3892/ijmm.2017.3185

    Figure Lengend Snippet: Validation of sequencing results by RT-qPCR.

    Article Snippet: LncRNA high throughput sequencing analysis, including labelling, hybridization, scanning, normalization and data analysis, was performed by Annoroad Geomics (Beijing, China) ( , ).

    Techniques: Biomarker Discovery, Sequencing, Next-Generation Sequencing